Summary
This report considers the scale-up of a process from laboratory scale to commercial scale for the production of alkaline phosphatase (AP) via the disaggregation and simulation of bovine liver tissue. The main stages of the process are collection, harvesting, chopping, disaggregation, stimulation and purification. However, certain process options are considered where some of these stages are removed, as per the table below. A financial model was developed by estimating the operating and capital expenses associated with the commercial scale process.
